Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific HEPES Buffered Saline Solution 2X, 500 Milliliters
HEPES Buffered Saline Solution 2X is a sterile, concentrated buffer solution used in biological and biochemical applications where stable pH control is required. The 2X concentration provides a convenient way to dilute the solution to the desired pH range for specific experiments, such as protein analysis, enzyme assays, and cell culture maintenance. HEPES is a widely used buffer in cell and molecular biology due to its minimal interference with biological processes and its ability to maintain a stable pH in a variety of biological environments. The 500mL bottle size is ideal for routine laboratory use in large-scale research and clinical applications.
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660062.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 500 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a versatile biochemical reagent used to maintain pH stability in laboratory experiments. This 500g package is suitable for medium to high-volume buffer preparations required in molecular biology, protein analysis, and biochemical assays. MOPS is commonly used in electrophoresis, chromatography, and enzyme assays where maintaining a consistent pH in the range of 6.5 to 7.9 is essential. It is particularly useful in applications that involve the stabilization of proteins, enzymes, and nucleic acids, making it a critical reagent in molecular research, cell biology, and biochemistry laboratories.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255316.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Research Products International Corp Bicine Ultra-Pure, 250 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Medchemexpress LLC HEPES, NaCl, Sucrose, pH 7.2 buffer | 100 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily used to resuspend human erythrocyte ghost preparations.
- Physiological buffer
- Composed of HEPES, NaCl, and Sucrose
- Adjusted to pH 7.2
- Used to resuspend human erythrocyte ghost preparations
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, particularly in the preparation of enzyme buffers.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Especially useful in the preparation of enzyme buffers
Medchemexpress LLC MES potassium (2-Morpholinoethanesulphonic acid potassium) | 39946-25-3 | 99.88% | 5 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with pH range 5.5-7.0.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration at varying temperatures.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Exhibits poor affinity with metal ions, making it suitable for studies involving such ions.
Research Products International Corp Tricine-SDS Sample Buffer 2X Solution, 50 ML
2X Formulation:
SDS: 8%
Glycerol: 20%
Coomassie Blue: 0.01%
Phenol Red: 0.01%
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set crystals, 100 g
BioPerformance Certified, pH 8.0, average Mw 141.8, Synonym: Tris crystals. The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 100G GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade , multicompendial
Kd Medical Inc TRIS HCL, 1M PH 6.8, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 6.8. One 500ml bottle.
United States Biological Corporation TRIS HCL BUFFER 1M PH7.5,500ML
Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.