Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 6X SSC Molecular Biology Grade Solution. 500mL, Sterile. 4 pack.
6X SSC Molecular Biology Grade Solution. 500mL, Sterile. 4 pack.
Teknova 1X PBS with 200mM Sodium Chlor
1X PBS with 200mM Sodium Chloride, 0.001% P-188, 1000mL, Sterile 8 pack.
IBI SCIENTIFIC 3P 1X PBST (PBS W/0.05% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.05% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 1X PBST (1X PBS) with TWEEN 20 (0.05% TWEEN 20) is a ready-to-use sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 1X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
Kd Medical Inc TRIS HCL 1M PH 8.O 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 8.0. One 500ml bottle.
Crystalgen Inc PBS PH7.2 10X 500ML
PBS (Phosphate Buffered Saline) pH7.2 10X, with Ca, Mg-500mlUltra pure Phosphate buffered isotonic saline. With calcium and magnesium. Sterile filtered. 1X PBS solutions contains 137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 2mM KH2PO4.pH: 7.2Storage : 18-26oC
Crystalgen Inc PBS PH7.2 10X 12 X 500ML
PBS (Phosphate Buffered Saline) pH7.2 10X, with Ca, Mg-500mlUltra pure Phosphate buffered isotonic saline. With calcium and magnesium. Sterile filtered. 1X PBS solutions contains 137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 2mM KH2PO4.pH: 7.2Storage: 18-26oC
Dojindo Molecular Technologies Inc BES (100G), Good's buffer, CAS: 10191-18-1, pka=7.15, Active pH 6.6-8, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Dojindo Molecular Technologies Inc ADA (25G), Good's buffer, CAS: 26239-55-4, pka=6.6, Active pH 5.8-7.4, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Hampton Research HR2-587/0.5 M MES monohydrate - 100 ml
HR2-587/0.5 M MES monohydrate - 100 ml. Please note items from this supplier are non-returnable.
Hampton Research HR2-579/1.0 M TRIS hydrochloride - 100 ml
HR2-579/1.0 M TRIS hydrochloride - 100 ml. Please note items from this supplier are non-returnable.
Hampton Research HR2-723/1.0 M BICINE pH 9.0 - 100 ml
HR2-723/1.0 M BICINE pH 9.0 - 100 ml. Please note items from this supplier are non-returnable.
