Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TE (Tris-EDTA) Buffer, Sterile, 100 mL
Product DescriptionTE (Tris-EDTA) is a sterile buffer that has a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse activity is detected.TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 4x1L
Tris Buffered Saline-Tween TBST (10X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)
Crescent Chemical Co Inc EDTA ANALYTICAL GRADE
Ethylenediamine tetraacetic acid analytical grade Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 11278.02 CAS 60-00-4
Kd Medical Inc TRIS HCL, 1M, PH 7.5, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 7.5. One 500ml bottle.
Biotang Inc Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml
Dialysis tubing Storage buffer in PBS and Glycerol (autoclaved),; 125 ml.
IBI SCIENTIFIC 3P (EDTA) SOLUTION, 100 mL
IBI 0.5MEDTA solution (pH 8.0)is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions. Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic Acid | 60-00-4 | MFCD00003541 | 100g
Ethylenediaminetetraacetic Acid | Purity: 98% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 100g
Abcam Opti-Klear™ Live Cell Imaging Buffer 1 x , 60ML
Hepes based imaging buffer formulated to lower background fluorescence and maintain proper pH and osmolarity for hours at 37°C. ab275938 provides an energy source and preserves fluorescent signals.
The product is subject to the following: Abcam Restricted Use Statement
Mitegen Bis-Tris/ HCl, 1M, pH8.0, 250 ml, CAS # 6976-37-0
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Rockland Immunochemicals 1.0 M Tris HCl pH 8.5 - MB-027-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G